Coding

Part:BBa_K4309001:Design

Designed by: Yanqiu Tian   Group: iGEM22_NPU-CHINA   (2022-09-30)


Lignin peroxidase (LiP)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 571
    Illegal PstI site found at 361
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 571
    Illegal PstI site found at 361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 571
    Illegal BamHI site found at 915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 571
    Illegal PstI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 571
    Illegal PstI site found at 361
    Illegal NgoMIV site found at 483
    Illegal NgoMIV site found at 495
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A threshold value of IP was found for each enzyme: LiP oxidizes PAHs with an IP ≤7.55 eV , while MnP oxidizes PAHs with an IP up to 8.2 eV.


Source

synthetic

References